Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------------------------------------------------------------------------------------------------------------------MIPIPSAIDGQSFLLQELEQVMKPLGYVINGGWEYD---HGYFDYKIDDRDGYLFLRIPVNAVQGSLDERGAAVRIGTPFMLRQVFQADVDDHAEGGPF--QSLFNQFSE-----PERRDAEIDPAFLDIGASLVKELEDVLLH----------------------------------------------------------------------------------- 2W0C Chain:L ((1-335)) MIVKKKLAAGEFAETFKNGNNITIIKAVGELVLRAYGADGGEGLRTIVRQGVSIKGMNYTSVMLHTEYAQEIEYWVGDLDYSFQEQTTKSRDVNSFQIPLRDGVRELLPEDASRNRASIKSPVDIWIGGENMTALNGIVDG---------------GRKFEAGQEFQINTFGSVNYWVSDEEIRVFKEYSARAKYAQNEGRTALEANNVPF-----FDIDVPPELDGVPFSLKARVRHKSKGVDGLGDYTSISVKPAFYITEGD---ETTDTLIKYTSYGSTGSHSGYDFDDNTLDVMVTLSAGVHRVFPVETELDYDAVQEVQHDWYDESFTTFIEVYSDDPLLTVKGYAQILMERT

General information: TITO was launched using: RESULT: Template: 2W0C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 345 6048 17.53 54.48 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain L : 0.56 3D Compatibility (PKB) : 17.53 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.56 QMean score : -0.054

(partial model without unconserved sides chains): PDB file : Tito_2W0C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W0C-query.scw PDB file : Tito_Scwrl_2W0C.pdb: