Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKRKIGLAMSLVIAAGTILGACGNSEKSSGSGEGKNKFSVAMVTDVGGVDDKSFNQSAWEGIQAFGKENGLKKGKNG-YDYLQSKSDADYTTNLNKLARE-NFDLIYGVGYLMEDSISEIADQRKNTNFAIIDAVVD--KDNVASITFKEQEGSFLVGVAAALSS--------KSGKIGFVGGMESELIKKFEVGFRAGVQAVNP----KAVVEVKYAGGFDKADVGKATAESMYKSGVDVIYHSAGATGTGVFTEAKNLKKEDPKRDVWVIGVDKDQYAEGQVEGTDDNVTLTSMVKKVDTVVEDVTKKASDGKFPGGETLTYGLDQDGVGISPSK---------------------------------QNLSDDVIKAVDKWKKKIIDG-LEIPATEKE--LKTFKAE 4PEV Chain:A ((52-408)) -------------------------------------PISVLVLFDVGGRGDLSFNDMAALGADRAAEEL------GVDVVFQTPQSLAVMESVLDAASRSGEYDLIVLVGFLWQEPLEKVAPRYPEQKYALIDAATRERYDNVASYLFREQEVASLVGIIAADIANNISKATGEEAKAGAVAGMDIPPLWRFHIGYLYGVQYYNQAMGTDVEMVWTYTGRFDDPTLGKTTAEQMLQQGVRVFYGVAGLTHVGMFNAVKEAAAR--GVIAFSIGQDASQEWY------DPQTIIISGLKRVDVAVYTAIKDVVEGRFRGGIV-SLGLKEGGLGLSDEEIIRYFAEIAAETGQLPEGLTPEKVVEIVMSQREKWISNDGWRLVEELKQKIISGEIKFVTPQDHDTYDSII--

General information: TITO was launched using: RESULT: Template: 4PEV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1833 22446 12.25 73.59 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 12.25 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.506

(partial model without unconserved sides chains): PDB file : Tito_4PEV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PEV-query.scw PDB file : Tito_Scwrl_4PEV.pdb: