Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKRFSLIMMTGLLFGLTSPAFAAEKTETEAKAPANVAVLLDASGSMAKRIDGVSKFNSAKKEISKFASSLPEGTQVKMSVFGSEGNNKNSGKVQSCEAIRNVYGFQSFNEQSFLNSLNTIGPTGWTPIAKALNEAKSSFDQLDAKGEKVVYLLTDGEETCGGNPIKTAKELQ--KDNITVNVIGFDYKEGYKGQLNAIAKVGGGEYFPAYTQKDVEKIFTQQSLMLSK 4FX5 Chain:A ((76-256)) --------------------------------SENVEVIIIDCSGSMDYP---RTKMMAAKEATKVAIDTLTDGAFFAVVAGTEGARVV-------YPTGGQLLRADYQSRAAAKEAVGRLHANGGTAMGRWLAQAGRIFDTA-PSAIKHAILLTDGKDESE-TPADLARAIQSSIGNFTADCRGIGEDW-EPKELRKIADALLGTVGIIRDPATLAEDFREMTAK---

General information: TITO was launched using: RESULT: Template: 4FX5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 980 -25456 -25.98 -142.21 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -25.98 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.437

(partial model without unconserved sides chains): PDB file : Tito_4FX5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FX5-query.scw PDB file : Tito_Scwrl_4FX5.pdb: