Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAYPNGKLSEEKVFKDPVHRYVHVRDKLIWDLIGTREFQRLRRIKQLGTTYLTFHGAEHSRFNHSLGVYEIVRRMVDDVFKGRPE--WDDSERELCLAAALLHDLGHGPFSHSFEKVF--------HLDHEDFTRGIILGD---TEVNQVLRKVSP---GFPQDVAEVIAKTY----------KNKQVVSLISS---QIDADRMDYLQRDAYYTGVSYGHFDMERILRVMRPRE----DQIVIKESGMHAVEDYIMSRYQMYWQVYFHPVTRSAEVILTKILHRAKQLHDEGYVFTHAPVHFYSIFEGKLTLEDYVKLDESIILYYFQAWEDEEDAILSDLCRRFINRQLFQYVEFNPNEEMSAY-----FELT-SLF-------KEAGIDPSYYLVVDSSSDLPYDFYRPGEEEERLPIHLLTQNG--QIKEL--SRQSAIVESISGKRRTDHKLYYPKDLICDGTKHPEAKMKIRQLLGLT 4MZ7 Chain:A ((26-487)) --------DTMKVINDPIHGHIELH-PLLVRIIDTPQFQRLRYIKQLGGGYYVFPGASHNRFEHSLGVGYLAGCLVHALGEKQPELQISERDVLCVQIAGLCHDLGHGPFSHMFDGRFIPLARPEVKWTHEQGSVMMFEHLINSNGIKPVMEQYGLIPEEDIYFIKEQIVGPLELWPYKGRPENKSFLYEIVSNKRNGIDVDKWDYFARDCHHLGIQ-NNFDYKRFIKFARVCEVDNELRICARDKEVGNLYDMFHTRNSLHRRAYQHKVGNIIDTMITDAFLKADDYIEITG----AGGKKYRISTAIDDMEAYTKLTDNIFLEILYSTD-PKLKDAREILKQIEYRNLFKYVGETQPTGQIKIKREDYESLPKEVASAKPKVLLDVKLKAED-FIVDVINM-DYGM---QEKNPIDHVSFYCKTAPNRAIRITKNQVSQLLPEKFAE--QLIRVYCKKVDRKSLY---AARQYFVQWCA--

General information: TITO was launched using: RESULT: Template: 4MZ7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1977 -38371 -19.41 -94.51 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -19.41 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.312

(partial model without unconserved sides chains): PDB file : Tito_4MZ7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MZ7-query.scw PDB file : Tito_Scwrl_4MZ7.pdb: