Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLHKERRIGRLSVLL-LLNEAEESTQVEELERDGWKVCLGKVGSMDAHKVVAAIETASKKSGVIQSEGYRESHALYHATMEALHGVTRGEMLLGSLLRTVGLRFAVLRGNPYESEAEGDWIAVSLYGTIGAPIKGLEHETFGVGINHI 4OKQ Chain:B ((4-149)) ---EKSVRIGRQALLLAMLDEGEEGAILDELRASNWRYCQGRVGAMEPQKIVAAIETAAKRHEVVDGSLYRDMHALYHAILEAVHGVTRGQVELGDLLRTAGLRFAVVRGTPYEQPKEGEWIAVALYGTIGAPVRGLEHEAVGLGINHI

General information: TITO was launched using: RESULT: Template: 4OKQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 715 -50477 -70.60 -348.12 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.89 3D Compatibility (PKB) : -70.60 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.89 QMean score : 0.561

(partial model without unconserved sides chains): PDB file : Tito_4OKQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OKQ-query.scw PDB file : Tito_Scwrl_4OKQ.pdb: