Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLISWNVNGLRAVMRKMDFLSYLKEEDADIICLQETKIQDGQV--DLQ--PEDYHVYWNYAVKKGYSGTAVFSKQEPLQVIYGIGVEEHDQEGRVITLEFENVFVMTVYTPNSRRGLERIDYRMQWEEALLSYILELDQKKPVILCGDLNVAHQEIDLKNPKANRNNAGFSDQEREAFTRFLEA-GFVDSFRHVYPDLEGAYSWWSYRAGARDRNIGWRIDYFVVSESLKEQIEDASISADVMGSDHCPVELIINI 5DFF Chain:B ((20-276)) LKICSWNVDGLRAWIKKKG-LDWVKEEAPDILCLQETKCSENKLPAELQELPGLSHQYWSAPS---YSGVGLLSRQCPLKVSYGIGDE-----GRVIVAEFDSFVLVTAYVPNAGRGLVRLEYRQRWDEAFRKFLKGLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFGELLQAVPLADSFRHLYPNTPYAYTFWTYMMNARSKNVGWRLDYFLLSHSLLPALCDSKIRSKALGSDHCPITLYLAL

General information: TITO was launched using: RESULT: Template: 5DFF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1351 -14468 -10.71 -59.54 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain B : 0.86 3D Compatibility (PKB) : -10.71 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.624

(partial model without unconserved sides chains): PDB file : Tito_5DFF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DFF-query.scw PDB file : Tito_Scwrl_5DFF.pdb: