Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKYMSLLTRIEEMMQSTSYQEGDRLPSIRQLSARYQVSKSTVIRALQELEKRHLIYSVPKSGYYIVKKSGKSKSGQPGPIDFATSAPDPDVFPYLDFQHCINKAIDTYKNDLFIYGTPKGLPSLIRVLRKLLAT-QQVF-ADERHIFITSGVQQALSLLCAMPFPNGKEKIAIEQPGYHLMVEQLETLGIPAIGVKRTEEGLDIAKVERLFQT-------ESIKFFYTMPRFHNPLGCSLSERDKQELVRLAEAYDVYLVEDDYLGDLEENKK-ADPLYAYDLSSHVIYLKSFSKMMFPGLRVGAAVLPEALTDTFYAYKKLNDIDCSMISQAALEIYLKSGMYGRHKEKIRDSYKERSLRLHQAIRTHRQLGS--GRFTFSSGQAPCMHTHLVLPQDLPASRVIHRLEKQGVLLEAIDRHYLSDYPKENLLKINISNVKTEDIERGVKLLMSHL 1VP4 Chain:A ((42-415)) ----------------------------------------------------------------------------DKDAISFGGGVPDPETFPRKELAEIAKEIIEKEYHYTLQYSTTEGDPVLKQQILKLLERMYGITGLDEDNLIFTVGSQQALDLIGKLFL-DDESYCVLDDPAYLGAINAFRQYLANFVVVPLEDDGMDLNVLERKLSEFDKNGKIKQVKFIYVVSNFHNPAGVTTSLEKRKALVEIAEKYDLFIVEDDPYGALRYEGETVDPIFKIGGPERVVLLNTFSKVLAPGLRIGMVAGSKEFIRKIVQAKQSADLCSPAITHRLAARYLERYDLLEQLKPTIELYRRKRTVMLNALEE--YFSDIPG-VKWVK-SEGGLFIWLTLPEGFDTWEMFEYAKRKKVFYVPGRVFKVYDE-PSPSMRLSFCLPPDEKIVEGIKRLREVV

General information: TITO was launched using: RESULT: Template: 1VP4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2081 -255963 -123.00 -707.08 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -123.00 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.543

(partial model without unconserved sides chains): PDB file : Tito_1VP4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VP4-query.scw PDB file : Tito_Scwrl_1VP4.pdb: