Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKMLYTLGGWVARNRIKAICAWIVVLVAAIGLAVTLKPSFSEDMSIPDTPSEKAMDVIQKEFPHGPDKGSIRVIFGAGDGEKLTGKPAKKAIEDTFKEISKDDSVDSIASPFVTGTIAKDGTVAYADIQYKSSADDIKDYSIKHLKDSLKMADDEGLQTELSGDVPGAEMEIGGVSEIVGIILAFVVLAITFGSLLIAGLPILTALIGLGVSIGLVLIGTQVFDIASVSLSLAGMIGLAVGIDYALFIFTKHRQFLGEGIQKNESIARAVGTAGSAVVFAGLTVIVALCGLTVVNIPFMSAMGLTAGLSVLMAVLASITLVPAVL-----SIAGKR------MIPKSNKKIEKQSTETNVWGRFVTKNPIMLSVCSILILIVISIPSMHLELGLPDAGMKAKDNPDRRAYDLLAEGFGEGFNGQLTIV-ADATNATENKAEAFADAVKEIKGLDHVASVTPAMPNKEGNFAIITVVPETGPNDVTTKDLVHDVRSLSDKNGVDLLVTGSTAVNIDISDRLNDAIPVFAVLIVGFAFVLLTIVFRSLLVPLVAVAGFMLTMTATLGICVFVLQDGNLIDFFKIPEKGPILAFLPILSIGILFGLAMDYQVFLVSRMREEYVKTNNPVQAIQAGLKHSGPVVTAAGLIMIFVFAGFIFAGEASIKANGLALSFGVLFDAFIVRMTLIPSVMKLMGNAAWYLPKWLDKIIPNVDIEGHQLTKEIQPEIDHEQKKQISV 1ZRO Chain:A ((8-601)) NEVLSNC---REKRKGMKWDCKKKNDRSNYVCIPDRRIQLCIVNLAIIKTYTKETM---KDHFIEASKKESQLLLKKNDN-------KYNSKFCNDLKNSFLDYGHLAMGNDMDFGGYSTKAENKIQEV-FKGAHGEISEHKIKNFRKKWWNEFREKLWEAML-----SEHKNNICKNIPQEELQITQWIKEWHGEFLLERDNRAKLPKSKCK------NNALYEACE---------------KECIDPCMKYRDWIIRSKFEWHTLSKEYETQKVPKENAENYLIKISENKNDAKVSLL-LNNCDAEYSKYCDCKHTTTLVKSVLNGNDNTIKEKREHIDLDDFSKFGCDKNSVDTNTKVW---ECKKPYKLSTKD------VCVPPRRQELCLGNIDRIYDKNLLMIKEHILAIAIYESRILKRKYKNKDDKEVCKIINKTFADIRDIIGGTDYW-------------------------NDLSNRKLVGKINTNSNY------VHRNKQNDKLFRDEWWKVIKKDVWNVISWVFKDKTVCKEDDIENIPQFFRWFSEWGDD-----YCQDKTKMIETLKVECCED----------DNCKRKCNSYKEWI-SKKKEEYNKQAKQYQEYQKGNNYK-------------------------------------MYSEFKSI------------KPEVYLKKYSEKC-SNLNFED-EFKEELHSDYKNKCTMCPEV

General information: TITO was launched using: RESULT: Template: 1ZRO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2385 -135781 -56.93 -235.73 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -56.93 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.201

(partial model without unconserved sides chains): PDB file : Tito_1ZRO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZRO-query.scw PDB file : Tito_Scwrl_1ZRO.pdb: