Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTVLILNFPAEGHVNPTLGITKAFSDKGYDVHY--ISTEKYKKRL-----EAAGATVHLHRDLLRTTPIHVGSPNGILDFVKIHIKTSLDILQI-VKDLSK--SIQFDFVYYDKFG-AGELVRDYLDIPGVSSSASFLFGEEHLKILP-LHP--E----S--GAPLELDQ-----ECE----------DLLAKMKETYGVAPKNLVQFMNNKGELNVVYTSRYFQPE-----SDRFGDECLFIGPSFPKRAEK--------TDFPIEQLKDEKVIYISMGTVLDHTEDFFNLCIDAFSGFNGKVVIAAGEKADLT---K-LKQAPENFIIAPYVPQLEVLEQ--SDVFITHGGMNSVNEGIHFSVPLVVMPHDKDQPMVAQRLSE-LHAGYVISKDEVNAQILKQAVDEVLRN---DQYTAGIKKINQSFKECMDME-EVMERIDELIRQKNK 4REL Chain:A ((4-441)) KQHVAIFPFPFGSHLPPLLNLVLKLAHIAPNTSFSFIGTHSSNAFLFTKRHIPNNIRVFTISDGI---PEGHVPANNPIEKLDLFLSTGPDNLRKGIELAVAETKQSVTCIIADAFVTSSLLVAQTLNVPWIAFWPNVSCSLSLYFNIDLIRDKCSKDAKNATLDFLPGLSKLRVEDVPQDMLDVGEKETLFSRTLNSLG--------VVLPQAKAVVVNFFAELDPPLFVKYMRSKLQSLLYVVPLPCPQLLLPEIDSNGCLSW-LDSKSSRSVAYVCFGTVVSPPPQEVVAVAEALEESGFPFVWALKESLLSILPKGFVERTSTRGKVVSWVPQSHVLSHGSVGVFVTHCGANSVMESVSNGVPMICRPFFGDQGIAARVIQDIWEVGVIVEGKVFTKNGFVKSLNLILVQEDGKKIRDNALKVKQIVQDAVGPHGQAAEDFNTLVE----

General information: TITO was launched using: RESULT: Template: 4REL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2055 -282076 -137.26 -744.26 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -137.26 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.501

(partial model without unconserved sides chains): PDB file : Tito_4REL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4REL-query.scw PDB file : Tito_Scwrl_4REL.pdb: