Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEISDIIYGQHHID-GVLEELIKSAPVQRLKGIYQGGASFLVNRKWNVTRYEHSIGVMLLIKKLGGTI--------------------EEQIAGLLHDVSHTAFSHVVDVVFENQAEDYHENIFQQVIVHSEIPDILQKHG-YHTEELLSDDSRW----TLLEQPAPELCADRTDYTLRDMYRYGHIN-LHEAQTFLDHLIVRNGRMFPD--SIEAAEWFVSVYYKEVIDFFLNPVNVYGYEYLARALKAALRHD--------------------VISAEDLL-KTDQEVLNILRASK---NEEVLSLLTSIHPGIQVIEDDIQYDFHQKKKMRLIDPSIFLDDKWIKSSGVSEKVRKMGEAAYQKAKKGVYIKILKQ 4LRL Chain:A ((38-355)) KVFRDPVHNYIHVQHQVILDLINSAEVQRLRRIKQLGTSSFTFHGAEHSRFSHSLGVYEITRRICEIFQRNYSVERLGENGWNDDERLITLCAALLHDVGHGPYSHTFEHIFDTNHEAITVQIITSP--ETEVYQILNRVSADFPEKVASVITKQYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYKGGIAFAMNGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILDHLLHRAKELFENPEFDYDLQASLLVPFFKGDFTLQEYLKLDDGVLSTYFTQWMDVPDSILGDLAKRFLM----------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4LRL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1237 -134533 -108.76 -519.43 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -108.76 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.366

(partial model without unconserved sides chains): PDB file : Tito_4LRL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LRL-query.scw PDB file : Tito_Scwrl_4LRL.pdb: