Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKTSVRDIALEALIKLEQNQAYSNLLLKSVIKSNELSDQNRGLLTELVYGTLQNKIALDYMLKPFINKPQKVKPWVIQLLRLSLYQMEYLEKIPDRAAIHEAVEIAKIRGHKGIASFVNGVLRSIQREGVPSFDAIEDPVRRLATETSHPEWLVKEWADAYGFEAAEKICRIHLIPPKQTLRVNQMKADRAELLDQMAAEGIE-----------V-----EKGDLAE-D---AVKLLKGTIAGT-----HFFQNGEVSIQDESSMLVARALDPKSDETVLDACAAPGGKSAHIAELMKNKGSVTSLDLHKHKVKLIKEAADRLGL------TIIHAETMDARKAGETFENEQFDRILVDAPCSGFGVIRRKPD--MKYTKKPDDSARLAEIQLSILREIAPLVKKGGTLVYSTCTMDRTENDEVIHAFIQEHP---DFEP--DLSLEKRL-PEK---VRPFVRDGRLQILPHY-FGTDGFFICSMRKKG 4FZV Chain:A ((23-359)) ---------------------------------------------------------------------------------------------------------------------------------------------------------FDMTYSVQFGD-LWPSIRVSLLSEQKYGALVNNFAA-WDHVSAKLEQLSAKDFVNEAISHWELQSAAPSPASWACSPNLRCFTFDRGDISRFPPARPGSLGVMEYYLMDAASLLPVLALGLQPGDIVLDLCAAPGGKTLALLQTGC-CRNLAANDLSPSRIARLQKILHSYVPEEIRDGNQVRVTSWDGRKWGE-LEGDTYDRVLVDVPCTTDRHSLHEEENNIFKRSRKKERQILPVLQVQLLAAGLLATKPGGHVVYSTCSLSHLQNEYVVQGAIELLANQYSIQVQVED-LTHFRRVFMDTFCFFSSCQVGELVIPNLMANFGPMYFCKMRRLT

General information: TITO was launched using: RESULT: Template: 4FZV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1578 -146464 -92.82 -506.79 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -92.82 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.393

(partial model without unconserved sides chains): PDB file : Tito_4FZV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FZV-query.scw PDB file : Tito_Scwrl_4FZV.pdb: