Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVLCDDERSAFLLVLDERLVDFDSQGGNHISVYLVTHFELSDQSYKDVLSFNDDLLGMEHNCSYAMD-----------------ILSVKEELDFDFPFNMLAIKSYVQELIKMLGI-DITLPEMKERDFDKLSQN 1HLV Chain:A ((1-131)) ---MGPKRRQLTFREKSRIIQEVEENPDLRKGEIARRFNIPPSTLSTILKNKRAILASERKYGVASTCRKTNKLSPYDKLEGLLIAWFQQIRAAGLPVKGIILKEKALRIAEELGMDDFTASNGWLDRFRRRRS-

General information: TITO was launched using: RESULT: Template: 1HLV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 258 -33076 -128.20 -292.71 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -128.20 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.356

(partial model without unconserved sides chains): PDB file : Tito_1HLV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1HLV-query.scw PDB file : Tito_Scwrl_1HLV.pdb: