Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIQDMILTLQKHWSSQGCVLMQAYDVEKGAGTMSPYTFLRSIGPEPWKVAYVEPSRRPADGRYGENPNRL----YQHHQFQVIIKPSPDNIQEL-YLDSLRALGIDPLEHDIRFVEDNWENPSLGCAGLGWEVWLDGMEITQFTYFQQVGGLECKPVSVEITYGIERLASYIQDKENVFDLEWTSGFTVKDLFMMAEYEHSVYTFETSDVDMLFQLFSTYEKEAIKQMDNGLVH--PAYDYVLKCSHTFNLLDAKGAISVTERTGYI--ARVRNLARKVAKTYYEEREKLGFPMLKGEGSSHE 3IZ3 Chain:D ((1-291)) -----MLQQPTGGYTTLE---QFAFTIRNDGTNATPTQFLQLLSYEATENELVKKTIPTPETHLPSARNVPGNVYIEDAITQALFGISAQNVNAHGYFSRLSALALPNTSARLGLDGVIYNSETINIPFYDPAAVANFAATYAKLGNASTPRYRADMIDIYAHVGLELAGTDAERAAGVMPVKRAKFDSWEGSLISLSRDVVNWKI----LAFLIDLCS-LEGEALRAFKTRNRDVFRMMLFIMSTAVAANVVNRKVTKRVDRVLEYIGVNSMRTAGRTATITYDLSRHEFAAKFLQLTFTRWN

General information: TITO was launched using: RESULT: Template: 3IZ3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1248 -21322 -17.08 -75.61 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain D : 0.68 3D Compatibility (PKB) : -17.08 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.229

(partial model without unconserved sides chains): PDB file : Tito_3IZ3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3IZ3-query.scw PDB file : Tito_Scwrl_3IZ3.pdb: