Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEHHKTYHSANIKTATGSLLIEGPVSPEDLAGYEFHKDLTAFRPPRE--QHEALVDIAGL-PEGRIIIARDGRTIVGYVTYLYPDPLERWSEGNMEDLIELGAIEVAPDYRGCAVGKTLLTVSMMDEQMENYIVMTTEYYWHWDLKGMKKDVWEYRKIMEKMMNAGGLVWFATDEPEISSHPANCLMARIGKNVSQESIEQFDRLRFYHRYMY 3EY5 Chain:A ((5-136)) ------------------IRFQPITTSDVQHYKFMEELLVESFPPEEYRELEHLREYTDRIGNFHNNIIFDDDLPIGFITYWDFD-----------EFYYVEHFATNPALRNGGYGKRTLEHLC-E------------FLKRPIVLEVERPVEEMAKRRINFYQRHGFTLWEKD---------------------------------------

General information: TITO was launched using: RESULT: Template: 3EY5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 471 -15426 -32.75 -119.58 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -32.75 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.329

(partial model without unconserved sides chains): PDB file : Tito_3EY5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EY5-query.scw PDB file : Tito_Scwrl_3EY5.pdb: