Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVTRITGLASGMDIDDIVSKLMQTERAPLDKLTQKKQTL-EWQRDSYREVNSKIKELQDYMSKNTLTYPSTYQSKTVTSSNESVLTATGSVSAPNSSS---TVEVASLATAATYKANNYTGYTQG-----DYNLAFNVVAPGETTAKTVNISVTSADTIDNVISKLNSSDLGVSAF-KDKIWNGTEYVETIAFSSKATGAGGSIQAADSATADFMSGQLGFSLDADNKLTAYKEGTNAKVTINGFE----MEKLTNNFTVNGVTYSI---KNTTAATGPVTTSVSTDVDGIY----------NQIKEFVDKYNELVDSLNEKLKEE---KYRDYTP-LTSEQKEAMSDKEVELWEEKAKSGLLRNDSSISTGTNQMRTDFYTQVNADGKTYQLTEFGITTSSAY-------QLRGHLEINEEKLKAKIAEDP---QGVANLFTSGTNDSNYSDKGIMKRITNTLR----STVKSIEAKAGNSTMGASSYSIG------KNLNSISTEITDMQDRLNTI----ENRYYSKFSAMDSAIQKMNEQASYLSQLLVQ 2BVL Chain:A ((1-543)) -----MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVEKYLKLKDINSLTDICIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVWIGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFRENLNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYS---KEIDELNTYIEESLNKITQNSGNDVRNFGEFKNGESFNLYEQELVERWNLAAASDILRISALKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEHFDMLDEEVQSSFESVLASK--SDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKDSYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMELGKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNISQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGAL

General information: TITO was launched using: RESULT: Template: 2BVL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2215 138458 62.51 283.72 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 62.51 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.144

(partial model without unconserved sides chains): PDB file : Tito_2BVL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2BVL-query.scw PDB file : Tito_Scwrl_2BVL.pdb: