Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQKYEKLEKIGEGTYGTVFKAKNRETHEIVALKRVRLDDDDEGVPSSALREICLLKELKHKNIVRLHDVLHSDKKLTLVFEFCDQDLKKYFDSCN-GDLDPEIVKSLLFQLLKGLGFCHSRNVLHRDLKPQNLLINRNGELKLADFGLARAFGIPVRCYSAEVVTLWYRPPDVLFGAKLYSTSIDMWSAGCIFAELANAGRPLFPGNDVDDQLKRIFRLLGTPTEEQWPAMTKLPDYKP-YPMYPATTSLVNVVPKLNATGRDLLQNLLKCNPVQRISAEEALQHPYFSDFCPP 4EK4 Chain:A ((4-293)) MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKI---------PSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVT-RRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWA-RQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKP

General information: TITO was launched using: RESULT: Template: 4EK4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) 54131 for 2213 contacts (24.5/contact) + 2D Compatibility (PS) -28511 + (NN) -1183 + (LL) 384 1D Compatibility (HY) 21200 + (ID) 750 Total energy: 45271.0 ( 20.46 by residue) QMean score : 0.009

(partial model without unconserved sides chains): PDB file : Tito_4EK4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EK4-query.scw PDB file : Tito_Scwrl_4EK4.pdb: