Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------MTTENQNPLVLDKNEEISQQKA-DDIRLQLRQEPEVKRLAQQIDVK-NQMELLEYGKEPAVEISKFSDR-------ILGMMKTTSVTDSGTMLTQLGKIMDR--------FDKNDFDEPKGLMAKIFKRGGSMIE-KIFKKYQTLG--GEIEKIHVEISK------YKDEMTKTNYTLDEMYENNIKY-----------YMELEKYVVAGQMKLEEMQSILPSYEEKAASGNQLAQMQLD-----TLRNGIQALEERVYDLDM----------------ARMVALQTAPQIRLLQRGNAKLIGKINSAFIITIPIFKNGIIQAVTVKRQKLVADSMSELDRRTNEMLKRNAENISSQS-VEIARMAGRP-SIDIETI--ESSWNTIVSGMQETKQIEEENKRLREDGARRIAQLQDNIKKAALQQ 3J9O Chain:B ((79-505)) VIDKLIDLQVNSIISNDEFRALEQEWLKVQEVCQEDYDNVEVSILDVKKEELQYDFERNLYDISSSDFFKKVYVSEFDQYGGEPYGAILGLYNFENTTNDIIWLTGMGMVAKNSHAPFIASIDKSFFGVKDLSEITHIKSFEALLEHPRYKEWNDFRNLDVAAYIGLTVGDFMLRQPYNPENNPVQYKLMEGFNEFVDYDKNESYLWGPASIHLVKNMMRSYDKTRWFQYI--RGVESGGYVKNLVACVYDNKGILETKSPLNVLFADYMELSLANIGLIPFVSEKGTSNACFFSVNSAKKVEEFVDGFDSANSRLIANLSYTMCISRISHYIKCVIRDKIGSIVDVESIQKILSDWISEFVTTVYQPTPLEMARYPFRNVSIEVKTIPGKPGWYSCKINVIPHIQFEGMNTTMTIDTRLEPELFGTNN-------

General information: TITO was launched using: RESULT: Template: 3J9O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1204 -62485 -51.90 -176.51 target 2D structure prediction score : 0.32 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -51.90 2D Compatibility (Sec. Struct. Predict.) : 0.32 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.158

(partial model without unconserved sides chains): PDB file : Tito_3J9O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3J9O-query.scw PDB file : Tito_Scwrl_3J9O.pdb: