Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKRTVKNIAEMVKGTLANPQYEQTVIHGVATDTRKLEQHQLFIPLKGERFDGHSFVEQAFEAGVAAVLWDRSVPNPPENHAVILVDDTLTALQQLAKAYLQELGTRVIGVTGSNGKTTTKDMIHAVLGTQYRVHKTGGNFNNHIGLPLTVLAMPENTEIAVLEMGMSAKGEIDLLSRLANPDAAVITNIGESHMQDLGSREGIAEAKLEIINGLKEDGVLIYIGDEPLLQNAY-S-CQTKTYGTGTHNDYQLQDVSQSEEGTHFTIKGIENTFFIPILGKHN---VMNAMAAIAAGAYFGIAPEDAAKGLSGLKVTGMRLELIKTDSGLSIINDAYNASPTSMKAAIQLTESLEGYGKKMLVLGDMLELGDLEETFHKECGAVISPDKIDRVFTYGKLGAFIAEGALKHFEKDRVSHYTEKKDLLQAVKENASKGDLILFKASRGMKLEEIVKDLIESPLS 3ZL8 Chain:A ((3-422)) -----------------------DLLTRRFVLNSKEVREGDVFVAVKGKRFDGHDFIDEALRNGAYAIIAERKTV---NSDRIFLVESSVDTLAKLAREKLGNFSGTVVGVTGSSGKTTTKEILYNLLKNKRSVFKTPGNMNTEYGLPLSILNDYKGEEILVLEMAASRPGDIAHLCKIAPPDVAVLLNVGSAHLEFFGTRERIMETKMEIIKHSKENAIAVTLFDDPDLRKEVPRYRNTLFFGKEG-GDSVLKDWWYYEGSTIAEFEAFDSLFTVKLSGYWNGGQLLNIAASLCVMRTLGETVDI--FDLASLKTVPGRFNVREK-KGVLIVDDTYNASPEAFQTSIEALLRFP--GKKFAVVGAMKELGERSKEFHEELGERLNV--LDGVYVFLSEPEA--EWIKSK---KIILKSDDPEKIAKDLATRVKKGDVVLFKASRAVRIERVLEMFEKE---

General information: TITO was launched using: RESULT: Template: 3ZL8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2475 -260526 -105.26 -627.77 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -105.26 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.537

(partial model without unconserved sides chains): PDB file : Tito_3ZL8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZL8-query.scw PDB file : Tito_Scwrl_3ZL8.pdb: