Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQDLYQLIGEKLNDIIPGEWTKIYLYAEVLDDSTMVLFHFRTPENNQIIYSQDIPSHYNVSKDIFKTLLRELRELFEELRTEHRNNNDDVWTNLTLTLDRSGEFQLDYNYDDILASELDGYERIAIWEYKNLGILPEDEDDKEFVISYLGL 4HQE Chain:A ((4-108)) MMEVCPYL-EETFKILGRSW------------NGLIINYLSRCNDCSAHFSDMKRDLKTITPRALSLKLSELAQ--WELVEKQIISTSPVQIIYVLT-------------------------------EKGKALAEALHPIEAWAQSYVDL

General information: TITO was launched using: RESULT: Template: 4HQE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 321 -41006 -127.74 -390.53 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -127.74 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.369

(partial model without unconserved sides chains): PDB file : Tito_4HQE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HQE-query.scw PDB file : Tito_Scwrl_4HQE.pdb: