Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------MNNIGEIIS-NFEGIIGALLGVIVTLILTHILKHF--GQIKFYIVDFEIYFKTDNDGWGT-NVMPSKDEAKQI---------EIHSQIEIYNGAEIPKVLREIKFCFYKNTNLIVSVTPDDKATTEEFAEFGYYRDK-LFNINLPSKQI-IAINIIKFLNEKETKQVKKCNRVYLEAKDHNGKMYKVFLGEF--- 2P3P Chain:A ((9-205)) AGELERCFLAMPESVLPIVTMEERNDLCRRAGHLSGFTHTASLESSLGGTVTFLLNRNFIRIQTSTVGEVFMRILPFSDSSSVICVVTTVLHPVADSRIDFYTTEW--KPLKTDRFWQQPRIEDFFLPHTDRQSYAYQAIYASLTPSYMQVSLSEESDTLSIRQTVTETLAEEEKPLAAI----FLSPEP---LVYRWQSGRFVRQ

General information: TITO was launched using: RESULT: Template: 2P3P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 689 19053 27.65 113.41 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 27.65 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.301

(partial model without unconserved sides chains): PDB file : Tito_2P3P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P3P-query.scw PDB file : Tito_Scwrl_2P3P.pdb: