Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSMAYEEYMRQLVVPMRRELTGAGFEELTTAEEVENFMEK----AEGTTLVVVNSVC-GCAAGLARPAATQAVLQNDKTPDNTVTVFAGQDKEATAKMREYFTGQEPSSPSMALLKGKEVVHFIPRHEIEGHDMEEIMKNLTAAFDAHC 3D22 Chain:A ((21-132)) -------------------LAGGNVHLITTKERWDQKLSEASRDGKIVLANFSARWCGPSR--QIAPYYIELSE---NYPS---LMFLVIDVDELSDFSASWE--IKATPTFFFLRDGQQVDK-----LVGANKPELHKKITAILD---

General information: TITO was launched using: RESULT: Template: 3D22.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 495 10272 20.75 96.00 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 20.75 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.539

(partial model without unconserved sides chains): PDB file : Tito_3D22.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3D22-query.scw PDB file : Tito_Scwrl_3D22.pdb: