Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLNLKQIEFCLDKIGDMFPHAECELVHSNPFELVVAVALSAQCTDALVNRVTKTLFQKYKRPEDYLAVPLEELQQDIKSIGLYRNKAKNIQKLSKMIIEDYGG---------EVPRDRDELVKLPGVGRKTANVVVSVAFGVPAIAVDTHVERVSKRLGICRWKDSVLEVEKTLMRKVPKE--DWSVTHHRLIFFGRYHCKAQSPRCAECPLLSLCREGQKRDKKGLVKR 4UNF Chain:A ((33-245)) ------LAWVKERLHEEYGDQD-PTPRRDPMHELISTILSQRTTHADEEAAYQELR-TLGDWDAITLAPTDAVAHAIRRSNYPESKAPRIQETLRRIKAAPGGYDLDFLRDLPVKDALKWLTDLPGVGVKTASLVLLFNYARPVFPVDTHVHRVSTRVGVIP-RMGEQAAHRALLALLPPDPPYLYELHINFLSHGRQVCTWTRPKCGKCILRERCDAYALY--------

General information: TITO was launched using: RESULT: Template: 4UNF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 873 -40343 -46.21 -199.72 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -46.21 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.504

(partial model without unconserved sides chains): PDB file : Tito_4UNF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UNF-query.scw PDB file : Tito_Scwrl_4UNF.pdb: