Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDIRTITSSDYEMVTSVLNEWWGGRQLKEKLPRLFFEHFQDTSFITSEHNSMTGFLIGFQSQ---------SDPETAYIHFSGVHPDFRKMQIGKQLYDVFIETVKQ-RGCTRVKCVTSPVNKVSIAYHTKLGFDIEKGTKTVNGISVFANYDGPGQDRVLFVKNI 4R3L Chain:A ((12-167)) FTLRNARMDDIDQIIKINRLTLPENYPYYFFVEHL-KEYGLAFFVAIVDNSVVGYIMPRIEWGFSNIKQLPSLVRKGHVVSIAVLEEYRRKGIATTLLEASMKSMKNDYNAEEIYLEVRVSNYPAIALYEKLNFKKVK---------VLKGYYADGEDAYLMARPL

General information: TITO was launched using: RESULT: Template: 4R3L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 632 -48652 -76.98 -333.23 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -76.98 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.597

(partial model without unconserved sides chains): PDB file : Tito_4R3L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R3L-query.scw PDB file : Tito_Scwrl_4R3L.pdb: