Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MFVMVLRIIL---LALFAYC-IYAVVKYVANPKRRLKLAQSKEHFYIIDEQNNTRKNFQLTYKGVLFEGEKHIPSKDHPLFIHTIFVWTESPEKLKHFSAKDFENIEEKVLERYPNCKIDWDQPIKLAKKAEER- 4N5H Chain:X ((1-315)) MADEEAMLAKVQASWAQTAARDKARYADERVPEDVHWETEYRYEQSADPQQTLNLYYPAKRRNATMPTVIDIHGGGWFYGDRNLNRNYCRYLASQGYAVMGMGYRLLPDVDLRGQIQDIFASLRWLSHFGPQRGFDLDHVLLTGDSAGGHLASLVACIQQSAELQELFGVSRVNFNFTLVALVCPVAEPSKLPEAAGDMSDMAAFYLDKLSGGDQALVDHLNFSQVVKGLDLPP-----------FMLIGGQNDS---FYLQSQALLKVFDAN-----HVTYTTKLWPASAGPHLKHVFNVQHWEW--PESIETNLEMLRTFDALSKQQDQAEENE

General information: TITO was launched using: RESULT: Template: 4N5H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 365 29567 81.01 271.26 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain X : 0.59 3D Compatibility (PKB) : 81.01 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.112

(partial model without unconserved sides chains): PDB file : Tito_4N5H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4N5H-query.scw PDB file : Tito_Scwrl_4N5H.pdb: