Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------MIPIPSAIDGQSFLLQE------------------LEQVMKPLGYVINGGWEYDHGYFDYKIDDRDGYLFLRIPV--------NAVQGSLDERGAAVRIGTPFMLRQVFQADVDDHAEGGPFQSLF-NQFSEPERRDAEIDPAFLDI------------GASLVKELEDVLLH--- 1D02 Chain:A ((6-202)) LSGRLNWQALAGLKASGAEQNLYNVFNAVFEGTKYVLYEKPKHLKNLYAQVVLPDDVIKEIFNPLIDLSTTQWGVSPA-FAIENTETHKILFGEIKRQDGWVEGKDPSAGRGNAHERSCKLFTPGLLKAYRTIGGINDEEILPFWVVFEGDITRDPKRVREITFWYDHYQDNYFMWRPNESGEKLVQHFNEKLKKYLD

General information: TITO was launched using: RESULT: Template: 1D02.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 373 7830 20.99 58.87 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 20.99 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.176

(partial model without unconserved sides chains): PDB file : Tito_1D02.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1D02-query.scw PDB file : Tito_Scwrl_1D02.pdb: