Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDIIFVCTGNTCRSPMAEALFKSIAEREGLNVNVRSAGVFASPNGKATPHAVEALFEKHIALNHVSSPLTEELMESADLVLAMTHQHKQIIASQFGRYRDKVFTLKEYVTGSHGDVLDPFGGSIDIYKQTRDELEELLRQLAKQLKKDRR 4PIC Chain:A ((3-149)) YRILFVCTGNTCRSPMAAALLENKQL---PGVEVKSAGVFAAEGSEASVHAKMVLKEKGIEAAHRSSQLKKEHIDWATHVLAMTSGHKDMIVERFPEAKDKTFTLKQFVSGTDGDIADPFGGPIEVYRAARDELETLIDRLAEKLQTEQL

General information: TITO was launched using: RESULT: Template: 4PIC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 781 -88848 -113.76 -604.41 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : -113.76 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.553

(partial model without unconserved sides chains): PDB file : Tito_4PIC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PIC-query.scw PDB file : Tito_Scwrl_4PIC.pdb: