Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLHKERRIGRLSVLL-LLNEAEESTQVEELERDGWKVCLGKVGSMDAHKVVAAIETASKKSGVIQSEGYRESHALYHATMEALHGVTRGEMLLGSLLRTVGLRFAVLRGNPYESEAEGDWIAVSLYGTIGAPIKGLEHETFGVGINHI 4OK9 Chain:B ((4-149)) ---EKSVRIGRQALLLAMLDEGEEGAILDELRASNWRYCQGRVGAMEPQKIVAAIETAAKRHEVVDGSLYRDMHALYHAILEAVHGVTRGQVELGDLLRTAGLRFAVVRGTPYEQPKEGEWIAVALYGTIGAPVRGLEHEAVGLGINHI

General information: TITO was launched using: RESULT: Template: 4OK9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 708 -53070 -74.96 -366.00 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.89 3D Compatibility (PKB) : -74.96 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.89 QMean score : 0.569

(partial model without unconserved sides chains): PDB file : Tito_4OK9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OK9-query.scw PDB file : Tito_Scwrl_4OK9.pdb: