Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGNTNGDSAFNKRTIAAALANYIDAGSIVAGSAGLSLWVSYLKLSDTQIGLLGALSANAISAAVGALLGGFLADKVGRKAVYTNSMLVYALGICLVLFGVNFPMLLSGYIIIGLSVGADITASWTIIAENAPKKNRARHCGVAQVAWAAGAVVVLLLSVLAGDLGLLGNKIVFAHLLVIALITYIL-RIRLPESDAWQTKNQPEEAQAEKPAVLNKTSYFDLLKPMYLKSILFLMGVYLVWNLAAGVMGFFMPYIYQQVGGVSANMANLLQMGLFIFTGLGVALIFMPFADKYRKT-VFG-IAAFMAVIGWTLFLLP-VEGLPILLLFIVVIGINNGAGQQANYQLWASEIFPTQYRASAQGLMFFLVRISIGIWSLFVPMIITNFGIGTMAAILLGCVTASMIIGLLFAPNTSGKSLEQIQEELYGSPQSQVKKGTESKIM 2GFP Chain:A ((25-360)) ----------------------------TIYIPAIADMARDLNVREGAVQSVMGAY--LLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAASAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDT--MWNWRACYLFLLVLCAGVTFSMARWMPETRPVDAPR-----------TRLLTSYKTLFGNSGFNCYLLMLIGG---LAGIAAFEACSGVLMGAVLGLSSMTVSILFIL-PIPAAFFGAWFAGRPNKRFSTLMWQSVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGML-FPLATSGAMEPFP-FLAGTAGALVGGLQNIGSGVLASLSAMLPQT---------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2GFP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1506 -228439 -151.69 -688.07 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -151.69 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.240

(partial model without unconserved sides chains): PDB file : Tito_2GFP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GFP-query.scw PDB file : Tito_Scwrl_2GFP.pdb: