Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTNNLKQRRIILDLAVTLDGFIEGKNGEVDWCIMDPDMGFTDFLNQIDTILYGRKSFDLWGQYIPKNEDPDTEKELWKLVHSKKKYVFSRTQNEIDNQAIFINDNILEEVNKLKKNPGKDIWLYGGASLITTFINLGLVDEFRLSIHPVVLGEGKPLFIDVKQRINLKMVNTRTFSSGVVQIVYHWNG 3IX9 Chain:A ((22-167)) ------TKKIVAIWAQDEEGVIG-KDNRLPWYLPAELQHFKETT-LNHAILMGRVTFDGMGRR---------------LLPKRETLILTRNPEEKIDGVATF-HDVQSVLDWYSA-QEKNLYIVGGKQIFQAFEP--YLDEVIVTHIHARV-EGDTYFPAEFDLSLFETVSSKF--------------

General information: TITO was launched using: RESULT: Template: 3IX9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 620 -89973 -145.12 -616.25 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -145.12 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.344

(partial model without unconserved sides chains): PDB file : Tito_3IX9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3IX9-query.scw PDB file : Tito_Scwrl_3IX9.pdb: