Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIRIGTRGSLLA---TTQAATVRDALIAGGHSAELVTISTEGDRSMAPIASLGVGVFTTALREAMEAGLVDAAVHSYKDLPTAADPRFTVAAIPPRND------------PRDAVVARDGLTLGELPVG--------------------SLVGTSSPRRAA----QLRALGLGLEIRPLRGNLDTRLNKVSSGDLDAIVV--ARAGLARLGRLDDVTETLEPVQMLP----APAQG--------ALAVECRAGD-------SRLVAVL---AELDDADTRAAVTAERALLADLEAGC----------SAPVGAIAEVVESIDEDGRVFEELSLRGCVAALDGS------------DVIRASGIGSCGRARELGLSVAAELFELG----ARELMWGVRH-- 4X4W Chain:A ((1-409)) MFTMKLQSPEFQSLFTEGLKSLTELFVKENH--ELRIAGGAVRDLLNGVKPQDIDFATTATPTQMKEMFQSAGIRMINGTITARLHEENFEITTLRIDVTTDGAEVEFTTDWQKDAERRDLTINSMFLGFDGTLFDYFNGYEDLKNKKVRFVGHAKQRIQEDYLRILRYFRFYGRIVDKPGDHDPETLEAIAENAKGLAGISGERIWVELKKILVGNHVNHLIHLIYDLDVAPYIGLPANASLEEFDKVSKNVDGFSPKPVTLLASLFKVQDDVTKLDLRLKIAKEEKNLGLFIVKNRKDLIKATDSSDPLKPYQDFIIDSDATTRVCELLKYQGEHCLLKEMQQWSIPPFPVSGHDIRKVGISSGKEIGALLQQLREQWKKSGYQMEKDELLSYIKKTL

General information: TITO was launched using: RESULT: Template: 4X4W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -50490 for 2088 contacts (-24.2/contact) + 2D Compatibility (PS) -33862 + (NN) -17481 + (LL) 116 1D Compatibility (HY) -9200 + (ID) 2700 Total energy: -113617.0 ( -54.41 by residue) QMean score : 0.221

(partial model without unconserved sides chains): PDB file : Tito_4X4W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4X4W-query.scw PDB file : Tito_Scwrl_4X4W.pdb: