Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------------------------------------------------------------------------------------------------------------------------------------MVNGIYTKSFLERIQEELPEWQRIAFELLAETLGDDADTFPCIPGRQAFLTDQLR-------IAFAGDPRENRTAEELAPLLAEYGKISRDTGKYASLVVLFDTPEDLAEHYSIEAYEELFWRFLNRLSHQDEKEWPEDIPADPEHYKWEFCFDGEPYFILCATPGHEARRSRSFPFFMVTFQPRWVFDDLNGSTAFGRNMSRLIRSRLEAYDQAPIHPQLGWYGGKDNREWKQYFLRDDEKQVSKCPFSYLKNMFNKMK--------------------------------------------------------------------------------------------------------------------------------- 1EZ0 Chain:A ((5-508)) TDNVFYATNAFTGEALPLAFPVHTEVEVNQAATAAAKVARDFRRLNNSKRASLLRTIASELEARSDDIIARAHLETALPEVRLTGEIARTANQLRLFADVVNSGSYHQAILDTPNPTRAPLPKPDIRRQQIALGPVAVFGASNFPLAFSAAGGDTASALAAGCPVIVKG-HTAHPGTSQIVAECIEQALKQE-QLPQAIFTLLQGNQRALGQALVSHPEIKAVGFTGSVGGGRALFNLAHERPEPIPFYGELGAINPTFIFPSAMRA-KADLADQFVASMTMG------------------------CGQFCTKPGVVFAL---------NTPETQAFIETAQSL--IRQQSPSTLLTPGIRDSYQSQVVSRGSDDGIDVTF--SQAESPCVASALFVTSSENWRK-----HPAWEEEIFGPQSLIVVCENVADMLSLSEMLAGSLTATIHATEEDYPQVSQLIPRLEEIAGRLVFNGWPTGVEVGYAMVHGGPYPASTHSASTSVGAEAIHRWLRPVAYQALPESLLPDSLKAENPLEIARAVDGKAA

General information: TITO was launched using: RESULT: Template: 1EZ0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 737 -53185 -72.16 -255.69 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -72.16 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.253

(partial model without unconserved sides chains): PDB file : Tito_1EZ0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EZ0-query.scw PDB file : Tito_Scwrl_1EZ0.pdb: