Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------------------------------------MNRLPFGYIKLRLAGRKANPMTNLIH-------------HWSGGSMNFTWESLVLIVAGVILLRISGRKS----IAQMTSTQTVVMISIGTIIVQPIIE--YSLFKT--LIAAAIFTSTLIVMEWI--------QMKSNTIEKMLTGKAKIVIENGQ--------------LHIENLKKMR----LTADQLEMQLRLHGVTAIQDVKIATLEANGQLGIELTDDAKPLTVRDLK---KLIHPDFIN-KDGQAQSGNQNIFDEVGKKNKKNVPKKLH 4CZX Chain:A ((1-324)) PHMMDSRDWTQLGCVAYPSPIHPDYHAGPASTIAFDNQDELLWIGTQKGFAGSFIGRELKRFTAFRIHPETDGPLRQFLFVDKGVIFLGSRSVYMAARSGVPIWSIRHESMQDLRAMSFTSKGTSEILVAGWQNKMLVIDVNKGEVVKELPTQDQYSFLKMSRYICAATNKGTVNILDPITFTIKKQWQAHGAFINDLDTSNDFIVTCGGSHRQTHNTPAILDPYVKVFDLKNMSAMNPVPFAPLAAHVRMHPR---MLTTAIVVNQAGQIHVTDLLNPSNSQVCYTQPQGVVLHFDVSRTGEGKALADNKHNTYVWGSPNKIQFTE---

General information: TITO was launched using: RESULT: Template: 4CZX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1027 -94264 -91.79 -430.43 target 2D structure prediction score : 0.34 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -91.79 2D Compatibility (Sec. Struct. Predict.) : 0.34 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.117

(partial model without unconserved sides chains): PDB file : Tito_4CZX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CZX-query.scw PDB file : Tito_Scwrl_4CZX.pdb: