Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTILAIDTSNYTLGIALLREDTVIAEYITYLKKNHSVRAMPAVHSLLNDCDMAPQDLSKIVVAKGPGSYTGVRIGVTLAKTLAWSLDIPISAVSSLETLAANGRH--FDGLISPIFDARRGQVYTGLYQYKNGLLEQVVPDQNVMLADWLEMLKEKDRPVLFLGHDTSLHKQMIEDVLGTKGFIGTAAQHNPRPSELAFLGKE----KEAADVHGLVPDYLRLAEAEAKWIESQK 5BR9 Chain:A ((3-216)) -TLLALDTSTEACSVALLHEGRALSHY-EVIPRLHAQRLLPMVRDLLDEAGVALSAVDAIAFGRGPGAFTGVRIAIGVVQGLAFALQRPVLAVSDLAILAQRAYREQGAERVAAAIDARMDEVYWGCYQLQQGEMRLAGSEAVLPPERVAVPWDAAAADWFGAGTGWGYVERM-PQ----RPVA-LDASLLPHAEDLLSLAGFAWARGEGVEAEQALPVYLR-------------

General information: TITO was launched using: RESULT: Template: 5BR9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1230 -136394 -110.89 -655.74 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -110.89 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.364

(partial model without unconserved sides chains): PDB file : Tito_5BR9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5BR9-query.scw PDB file : Tito_Scwrl_5BR9.pdb: