Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLKKVILAAFILVGSTLGAFSFSSDASAKHVNGNITWYNGVGKKGSSGKKLGHWDCATKIGFDVPRNGTKIRAYAKA--KPKKVITV---YKNDVGRMPNAVLDVSPKAFKALGYP--LSKGKVAGHYSY 3SUK Chain:A ((69-130)) -----------------------------------------------------------GGFNSPACGNCYTISF-TFQGVTRSINLVAIDHA----GNG--FNVAQAAMDELTNGNAVALGTIDVQS--

General information: TITO was launched using: RESULT: Template: 3SUK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 142 -24968 -175.83 -453.96 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -175.83 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.862

(partial model without unconserved sides chains): PDB file : Tito_3SUK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3SUK-query.scw PDB file : Tito_Scwrl_3SUK.pdb: