Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKYHISMINIPAYGHVNPTLALVEKLCEKGHRVTYATTEEFAPAVQQAGGEALIYHTSLNIDPKQIREM--MEKNDAPLSLLKESLSILPQLEELYK-DDQPDLIIYDFVALAGKLFAEKLNVPVIKLCSSYAQNESFQLGNEDMLK-K----IREAEAEFKAYLEQEKL-P--AVSFE--QLAVPEALNIVFMPKSFQIQHETF-DDRFCFVGPSLGERKEKESLLIDKDDRPLMLISLGTAFNAWPEFYKMCIKAFRDS-SWQVIMSVGKTIDPESLEDIPANFTIRQSVPQLEVLEKADLFISHGGMNSTMEAMNAGVPLVVIPQMYEQELTANRVDELGLGVYLPKEEVTVSSLQEAVQAVSSDQELLSRVKNMQKDVKEAGGAERAAAEIEAFMKKSAVPQ 2IYF Chain:A ((8-400)) ---AHIAMFSIAAHGHVNPSLEVIRELVARGHRVTYAIPPVFADKVAATGPRPVLYHSTLPGPDADPEAWGSTLLDN-VEPFLNDAIQALPQLADAYADDI-PDLVLHDITSYPARVLARRWGVPAVSLSPNLVAWKGYEEEVAEPMWREPRQTERGRAYY-A-RFE--AWLKENGITEHPDTFASHPPRSLVLIPKALQPHADRVDEDVYTFVGACQ----EGGWQRP-AGAEKVVLVSLGSAFT-QPAFYRECVRAFGNLPGWHLVLQIG---TPAELGELPDNVEVHDWVPQLAILRQADLFVTHAGAGGSQEGLATATPMIAVPQAVDQFGNADMLQGLGVARKLATEEATADLLRETALALVDDPEVARRLRRIQAEMAQEGGTRRAADLIEAELPA-----

General information: TITO was launched using: RESULT: Template: 2IYF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1982 -255811 -129.07 -693.25 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -129.07 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.586

(partial model without unconserved sides chains): PDB file : Tito_2IYF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2IYF-query.scw PDB file : Tito_Scwrl_2IYF.pdb: