Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------MKKAFILSAAAAVGLFTFGGVQQASAKELSCQPVVTVKTGNTVQNM------SLNDAVKKLHINTNIKTL--NAANEKEMKQLLQKHAKQSNVKVQDVQKTETAKPAQKTTEKAAA----DQNTASKAPATAEKTNTTTSAPSSVSA-YEKKVVELTNAE----RQKQGLKPLQIDETLSKSARAKSQDMK--DKNYFDHQSPTYGSP----FDMMKSFGISYKTAG----ENIAKGQKTPEEVVKAWMNSEGHRKNILNP---NFTHIGVGYVESGSIWTQQFIGK-- 4WBX Chain:C ((4-382)) RFPFPVGEPDFIQGDEAIARAAILAGCRFYAGYPITPASEIFEAMALYMPLVDGVVIQMEDEIASIAAAIGASWAGAKAMTATSGPGFSLMQENIGYAVMTETPVVIVDVQRSGL--PAQGDIMQAIWGTHGDHSLIVLSPSTVQEAFDFTIRAFNLSEKYRTPVILLTDAEVGHMRERVYIPNPDEIEIINRLIKRIVEKIEKNKKDIFTYETYELEDAVATGIVARSALLLKIETIWPFDFELIERIAERVDKLYVPEMNL-GQLYHLIKEGANGKAEVKLISKIGGEVHTPMEIFEFI

General information: TITO was launched using: RESULT: Template: 4WBX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1343 -44501 -33.14 -175.20 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain C : 0.64 3D Compatibility (PKB) : -33.14 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.154

(partial model without unconserved sides chains): PDB file : Tito_4WBX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WBX-query.scw PDB file : Tito_Scwrl_4WBX.pdb: