Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTYHPFSLTTPSTLMIQDWAQTNQNNKEVIAGFTTKNGGVSQKPFESLNTGLHVHDKDADVVKNREYIADMFNTDLQSWVFADQTHDNRVQKVTQRDRGKGAREYHTALKATDGIYTNEKNVFLALCFADCVPLFFYDPVKSLVGVAHAGWKGTVKQIGREMVKQWTEKEGSNPSDIYAVIGPSISGACYTVDDRVMDAVRALPVSADLAANQTAKAQYQLDLKELNRLILMDSGLASEQISVSGLCTESEPSLFYSHRRDQGKTGRMMSFIGMKEA 3OX7 Chain:P ((8-15)) -----------------------------------------------------------------------------------------------------------------------------------------------------CSWRGLEN-------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3OX7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain P - contact count / total energy / energy per contact / energy per residue : 1 -419 -419.00 -52.38 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain P : 0.53 3D Compatibility (PKB) : -419.00 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 1.166

(partial model without unconserved sides chains): PDB file : Tito_3OX7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OX7-query.scw PDB file : Tito_Scwrl_3OX7.pdb: