Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIIVQKFGGTSVKDDKGRKLALGHIKEAISEGYKVVVVVSAMGRKGDPYATDSLLGLLYGDQSAISPREQDLLLSCGETISSVVFTSMLLDNGVKAAALTGAQAGFLTNDQHTNAKIIEMK-PERLFSVLANHDAVVVAGFQGATEKGDTTTIGRGGSDTSAAALGAAVDAEYIDIFTDVEGVMTADPRVVENAKPLPVVTYTEICNLAYQGAKVISPRAVEIAMQAKVPIRVRSTYS-NDKGTLVTSHHSSKVGSDVFERLITGIAHVKDVTQFKV--PAKIGQYNVQTEVFKAMANAGISVDFFNITPSE--IVYTVAGNKTETA----QRILMDMGYDPMVTRNCAK----------VSAVGAGIMGVPGVTSKIVSALSEK--EIPILQSADSHTTIWVLVHEADMVPAVNALHEVFELSK 2HMF Chain:C ((107-466)) ------------------------------------------------------LIGVAYLGE--LTPKSRDYILSFGERLSSPILSGAIRDLGEKSIALEGGEAGIITDNNFGSARVKRLEVKERLLPLLKEGIIPVVTGFIGTTEEGYITTLGRGGSDYSAALIGYGLDADIIEIWTDVSGVYTTDPRLVPTARRIPKLSYIEAMELAYFGAKVLHPRTIEPAMEKGIPILVKNTFEPESEGTLITNDM------EMSDSIVKAISTIKNVALINIFGAGMVGVSGTAARIFKALGEEEVNVILISQGSSETNISLVVSEEDVDKALKALKREFGDF-----LNNNLIRDVSVDKDVCVISVVGAGMRGAKGIAGKIFTAVSESGANIKMIAQGSSEVNISFVIDEKDLLNCVRKLHEKF----

General information: TITO was launched using: RESULT: Template: 2HMF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1645 -196929 -119.71 -591.38 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain C : 0.76 3D Compatibility (PKB) : -119.71 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.450

(partial model without unconserved sides chains): PDB file : Tito_2HMF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HMF-query.scw PDB file : Tito_Scwrl_2HMF.pdb: