Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDTYSVFTTKWKQLTGVDLTLYKEAQMKRRLTSLYEKKGFQSFKDFAAALEKDQALLNETLDRMTINVSEFYRNYKRWEVLETAILPLIKTSRPLKIWSAACSTGEEPYTLAMLLDQQKGLPGYQILATDIDEKALEKAKKGVYQERSLQEVPLSVKDRYFTQNANRSYEVKTEIKKNITFKKHNLLADRYEQDFDLIVCRNVFIYFTESAKEELYLKMAHSLKKNGVLFVGSTEQIFNPEKFGLVPADTFFYQKR 5EPE Chain:A ((36-144)) --------------------------------------------------------------------------------------------AGARVLDLGSGSGEM----LCTWARDH---GIVGTGIDLSQLFTEQAKRRAEAL---------------------------GVAGQVKFIHGDAAGYVSDEKVDVAACVGASWIAGGV--AGTITLLAQSLEPGGIILMGEPFWR-------------------

General information: TITO was launched using: RESULT: Template: 5EPE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 549 -60173 -109.60 -552.04 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -109.60 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.439

(partial model without unconserved sides chains): PDB file : Tito_5EPE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EPE-query.scw PDB file : Tito_Scwrl_5EPE.pdb: