Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRNKRRNR-QIVVAVNGGKAVKAIFLFIVSLIVIFVLSGVLTSLRPELRPSSDSFYGIAEELPGDVFA-HLLQMENHYFASDLSQTDSSFHLSRLSLKLATSINLEDPRSFLGRE-LPGFAQFDTEILLAGQGTDYTNMPAESPPPSKVMEEERE-ANLAEIEKQQTQSDNA-QKDPPKQTTGDKKVVFIYHTHNTES-YLPLLKGETDPDMARHSKANVTLVGDMFGQALESQGIGA-TVNKTDIQS-KLNKKGLNYARSY-DESRPVVKDALASNKNLQY-----IIDIHRDSRRKKDTTATIKGKSYARVAFVVGKK-SKNF-EENYKIASELHKLMEKKYPGLSTGV-FSKGSPGDNGVYNQDLTDRALLLEFGGVDNNLE-ELQRAANAAADVFSEMYWDAEKVNAASGE-TKKQ------- 3K2K Chain:A ((12-402)) HENLYFQGMTLSITSNFDAGAIDVVSCERADAIRLRVRGDNRSEFAQW------FYFRLTGARGERCVMTFENANDCAYPAGWRDYRAVASYDRVNWFRV--------PTSYDGQMLTIDHTPEFDSIHYAYFEPYS-----EERHSEFLGAVQQMPQASVVELGRTVEGRPMSLVVLGTPDAKKKVWIIARQHPGESMAEWFIEGLVK-RLVGWGDWSGDPVARKLYDHATFYIVPNMNPDGSVHGNLRTNAAGANLNREWMEPDAERSPEVLVVRDAIHAIGCDLFFDIHGDEDLPY--------------VFAAGSEMLPGFTEQQRVEQSAFIDSFKRASPDFQDEHGYPPGKYRED---AFKLASKYIGHRFGCLSLTLEMPFKDNANLPD---EHIGWNGARSASLGAAMLGAILEHVRAF

General information: TITO was launched using: RESULT: Template: 3K2K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1992 114079 57.27 316.01 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 57.27 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.113

(partial model without unconserved sides chains): PDB file : Tito_3K2K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3K2K-query.scw PDB file : Tito_Scwrl_3K2K.pdb: