Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVTIKKDFIPVSNDNRPGYAMAPAYITVHNTANTAKGADAKMHAKFVKNPNTSESWHFTVD--------DSVIYQHLPIDENGWHAGDGTN---GTGNRKSIGIEICENAD---------GDFEKATSNAQ-WLIRKLMKENNIPLNRVVPHKKWS--GKECPRKLLDHWNSFLNGISSSDTPPKETSPSYPLPSGVIKLTSPYRKGTNILQLQKALAVLHFYPDKGAKNNGIDGVYGPKTANAVKRFQLMNG-LTADGIYGPKTKAKLKSKLK 3D2Y Chain:A ((25-255)) ------------------AYPRIKVLVIHYTADDFDSS---LA-TLT---DKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGISAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKPENVVAHADIAPQRKDDPGPL-FPWQQLAQQGIGAWPDAQ-RVNFY-------LAGRAPHTPVDTASLLELLARYGYD-------VKPDM-TPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALL-

General information: TITO was launched using: RESULT: Template: 3D2Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 935 11670 12.48 56.37 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 12.48 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.399

(partial model without unconserved sides chains): PDB file : Tito_3D2Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3D2Y-query.scw PDB file : Tito_Scwrl_3D2Y.pdb: