Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAERVRVRVRKKKKSKRRKILKRIMLLFALALLVVVGLGGYKLYKTINAADESYDALSRGNKSNLRNEVVDMKKKPFSILFMGIEDYATKGQKGRSDSLIVVTLDPKNKTMKMLSIPRDTRVQLAGDTTGSKTKINAAYSKGGKDETVETVENFLQIPIDKYVTVDFDGFKDVINEVGGIDVDVPFDFDEKSDVDESKRIYFKKGEMHLNGEEALAYARMRKQDKRGDFGRNDRQKQILNALIDRMSSASNI----AKIDKIAEKASENVETNIRITEGLALQQIYSGFT---SKKIDTLSITGSDLYLGPNNTYYFEPDATNLEKVRKTLQEHLDYTPDTSTGTSGTEDGTDSSSSSGSTGSTGTTTDGTTNGSSYSNDSSTSSNNSTTNSTTDSSY 4OBM Chain:A ((17-257)) ---------------------------------------------------------------------TPDSTYDFSVLMTLSADN-----DKTPDKYMTITYIAKNNTFVLMPYLPNAVI--DG-----GNTIKQICEQSGEAEVAKLLSSKTGLSINKYIRFTKSTLTELFDMVGNTTLTVPSEIKYENKK-DNTVTIIKKGTQIFTAEQMYAYLTLPDYG----VKDELYPCKLNATVISSFIDQNFIGTSSKTLDEYINFIINFTNTNIEQSDYDAKVKAIVYTLSQNKSSVTDFYIPYGDK-----SGDDYIIDDNSWNSAKKALG------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4OBM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1071 -65371 -61.04 -279.36 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -61.04 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_4OBM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OBM-query.scw PDB file : Tito_Scwrl_4OBM.pdb: