Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNGQTPARHYYKKLVPSLILILNCIQFLSHPTKADPILLAFVFAVYLAFIWIIPYVASTAVSLSIFIGLWLLTDFFWAVSGQEQGAAYFLIVFLMIYAAFRLPSRLSLIFTACLIGGNILLLSSQGGSLNTIISNISIMLGLYVLFSSMRFRREARREAERNHAELAKMHVQLEHAHKELQKAHAELQEASVLSLRYAVLEERTRIARDIHDSIGHELTSVIVQLQSLPYILKS------SKEDSEKVIQNVLSV--ARECLQEVRSVVHQMGRSE----------SMVGL-TALRGLIHQVE---ERSG-----LH---VSLDTAGLSEESWPPNVSETIYRILQEALTNIIRHADAS--------HAAAVISNDKSHLYVTITDDGQFTGS---------------------LTYGFGLTGMKERAEKAGGSLTFSAVQPSGLKIELSLPLMTTNKEQKDEQR 2E0A Chain:A ((93-354)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QKALSDFVDTLIKVRNRHHNVVPTMAQGIIEYKDACTVDPVTNQNLQYFLDRFYMNRISTRML-MNQHILIFSDSQTGNPSHIGSIDPNCDVVAVVQDAFECSRMLCDQYYLSSPELKLTQVNGKFPD--QPIHIVYVPSHLHHMLFELFKNAMRATVEHQENQPSLTPIEVIVVLGKEDLTIKISDRGGGVPLRIIDRLFSYTYSTAPTPNAPLAGFGYGLPISRLYAKYFQGDLNLYSLSGYGTDAIIYLKALSSES-------

General information: TITO was launched using: RESULT: Template: 2E0A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 614 14601 23.78 74.11 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : 23.78 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.464

(partial model without unconserved sides chains): PDB file : Tito_2E0A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2E0A-query.scw PDB file : Tito_Scwrl_2E0A.pdb: