Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKEEIIERFTTYVKVDTQSDESVDTCPSTPGQ-LTLGNMLVDELKSIGMQDAAID-----------ENGYVMATLPSNTEKDVPTIGFLAHVDTATDFTGKNVNPQIIESYDGKDIVLNEQLQVTLSPDQFPELSGYKGHTLITTDGTTLLGADNKAGIAEIMTAMDYLIKHPEIKHGTIRVAFTPDEEIGR-GPHKFDVKR---F--NASFAYTVDGGPL--GELEYESFNAAAAKITIKGN--NVHPGTAKGKMINSAKIAMKLNSLLPADEA---------------------------P---------------------EYTEGYEGFYHLLSIQGD----------VEETKLHYIIRDFDKENFQNRKETMKRAVEELQNEYG-QDRILLDMNDQYYNMREKIEPVIEIVNIAKQAMEN-LGIEPKISPIRGGT--DGSQLSY---MGLPTPNIFTGGENFHGKFEYISVDNMVKAVNVIVEIAKQFEAQA 3DLJ Chain:A ((24-484)) -QDEFVQTLKEWVAIESDSVQPV---PRFRQELFRMMAVAADTLQRLGARVASVDMGPQQLGQSLPIPPVILAELGSD--PTKGTVCFYGHLDVQPADRGDGWL---TDPYVL-TEV----------------------DGKLYGRG----ATDNKGPVLAWINAVSAFRALEQDLPVNIKFIIEGMEEAGSVALEELVEKEKDRFFSGVDYIVISDNLWISKPAITYGTRGNSYFMVEVKCRDQDFHSGTFGGILHEPMADLVALLGSLVDSSGHILVPGIYDEVVPLTEEEINTYKAIHLDLEEYRNSSRVEKFLFDTKEEILMHLWRYPSLSIHGIEGAFDEPGTKTVIPGRVIGKFSIRLVPHMNVSAVEKQVTRHLEDVFSKRNSSNKMVVSMTLGLHPWIA--NIDDTQYLAAKRAIRTVFGTEPDMI-RDGSTIPIAKMFQEIVH--VVLIPLGAVDDGEHSQNEKINRWNYIEGTKLFAAFFLEMAQL-

General information: TITO was launched using: RESULT: Template: 3DLJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1629 37146 22.80 100.94 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 22.80 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.459

(partial model without unconserved sides chains): PDB file : Tito_3DLJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DLJ-query.scw PDB file : Tito_Scwrl_3DLJ.pdb: