Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKDGLCRADQQYECVAEIGEGAYGKVFKARDLKNGGRFVALKRVRVQTGEEGMPLSTIREVAVLRHLETFEHPNVVRLFDVCTVSRTDRETKLTLVFEHVDQDLTTYLDKVPEPGVPTETIKDMMFQLLRGLDFLHSHRVVHRDLKPQNILVTSSGQIKLADFGLARIYSFQMALTSVVVTLWYRAPEVLLQSS-YATPVDLWSVGCIFAEMFRRKPLFRGSSDVDQLGKILDVIGLPGEEDWPRDVALP--RQAFHSKSAQPIEKFVTDIDELGKDLLLKCLTFNPAKRISAYSALSHPYFQDLERCKENLDSHLPPSQNTSELNTA 4FKJ Chain:A ((5-292)) ----------ENFQKVEKIGEGTYGVVYKARN-KLTGEVVALKKI---------PSTAIREISLLKELN---HPNIVKLLDVIHT-----ENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARA----------VVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL--------------

General information: TITO was launched using: RESULT: Template: 4FKJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) 12681 for 2079 contacts (6.1/contact) + 2D Compatibility (PS) -26556 + (NN) 480 + (LL) 3068 1D Compatibility (HY) 18400 + (ID) 900 Total energy: 7173.0 ( 3.45 by residue) QMean score : 0.150

(partial model without unconserved sides chains): PDB file : Tito_4FKJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FKJ-query.scw PDB file : Tito_Scwrl_4FKJ.pdb: