Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------------------------------------MKRMIVRMTLPLLIVCLAFSSFSASARA-ASEEKYWDHWIERHAQPLDASNASNK---DLRFLKKVLKGK---RIV----QLGETTHGAGEINATKVRMIKY-----------------------LHEEL-----GYDVLAFESGFPDTNASYLNMDQLTPKSTMKNSIYAVW-----HTEDVVELFDYMKE-----------------------QKEKGDPLILTGFDIQS----MKNSFNVAAT------QWVKAVDPEKAE----LLSQSENDFST-----------------------LVTDSNTFDEFSQKKEKLVKNYQKLIKFTKTHASELKENLPKEPKAYEMFMHSL-----QLRIDVMETYMLEEMKEKLEE--------------YPENI----EDFSFFMRDRMMAEQFQWVADTLYPKKKIIVWGHNYHLR---------KQNTKMIKDWVQL---NGPNMGDYLPERLKKQTYTIGIYA---YSGASLDSSDNKTVKPVTSPPPSGSLEALLKA-ADRPAVFVDFLHTKNKK--GTSWMYTPR----TALYWGYMEEQMILKEQYDG--------VIWLEHIT--PSVIIK--- 4M1K Chain:A ((6-688)) EYDLKRLRNIGIAAHIDAGKTTTTERILYYTGRIAAVTTCFWKDHRINIIDTPGHVDFTIEVERSMRVLDGAIVVFDSSQGVEPQSETVWRQAEKYKVPRIAFANKMDKTGADLWLVIRTMQERLGARPVVMQLPIGREDTFSGIIDVLRMKAYTYGNDLGTDIREIPIPEEYLDQAREYHEKLVEVAADFDENIMLKYLEGEEPTEEELVAAIRKGTIDLKITPVFLGSALKNKGVQLLLDAVVDYLPSPLDIPPIKGTTPEGEVVEIHPDPNGPLAALAFKIMADPYVGRLTFIRVYSGTLTSGSYVYNTTKGRKERVARLLRMHANHREEVEELKAGDLGAVVGLKETITGDTLVGEDAPRVILESIEVPEPVIDVAIEPKTKADQEKLSQALARLAEEDPTFRVSTHPETGQTIISGMGELHLEIIVDRLKREFKVDANVGKPQVAYRETITKPVDVEGKFIRLTGGRGQYGHVKIKVEPLPR----GSGFEFVNAIVGGVIPKEYIPAVQKGIEEAMQSGPLIG--FPVVDIKVTLYDGSYHEVDSSEMAFKIAGSMAIKEAVQKGDPVILEPIMRVEVTTPEEYMGDVIGDLNARRGQILGMEPRGNAQVIRAFVPLAEMFGYATDLRSKTQGRGSFVMFFDHYQEVPKQVQEKLI

General information: TITO was launched using: RESULT: Template: 4M1K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1797 -99143 -55.17 -225.33 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -55.17 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.046

(partial model without unconserved sides chains): PDB file : Tito_4M1K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M1K-query.scw PDB file : Tito_Scwrl_4M1K.pdb: