Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIHLSILDQAPVSKGESPVTTLQHSVELAQLSEQWGYKRYWFAEHHSTKGLASTAPEIMIARIAAQTNTIRVGSGGVLLPQYSPFKVAETFRQLEALYPNRIDLGVGRSPGGTTKTRLALTDGVK--------------KSLTEFNRQLQDVSYFLTDSLP-PDHPY---AGIKAAPLI--GTAPELWVLGLGENSARRAAHQGIGYVFGHFINPER-GENAFRIYRESFRPSAHF-SNPSALFTIFVICAKTDEEAEELALSQDLWLLRVGKGL-D--SRVPSI--EEAK---A-HPYTASDKKLIEEN-RKRMVIGSPTTVKQQLLDLTGCYETNEIMVLCNVFDFEAKKESYERLAELFL 1EZW Chain:A ((3-344)) EVSFGIELLPD--------DKPTKIAHLIKVAEDNGFEYAWICDHYNN-----YSYMGVLTLAAVITSKIKLGPGITNPYTRHPLITASNIATLDWISGGRAIIGMGPGDK---ATFDKMGLPFPCKIPIWNPEAEDEVGPATAIREVKEVIYQYLEGGPVEYEGKYVKTGTADVKARSIQGSDIPFYMGAQGPIMLKTAGEIANGVLVNA-SNPKDFE-VAVPKIEEGAKEAGRSLDEIDVAAYTCFSIDKDEDKAIEATKIVVAFIVMGSPDVVLERHGIDTEKAEQIAEAIGKGDFGTAIGLVDEDMIEAFSIAGDPDTVVDKIEELL-KAGVTQVVVGSPIG--PDKEKAIELVGQEVI

General information: TITO was launched using: RESULT: Template: 1EZW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1764 -156235 -88.57 -503.98 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -88.57 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.461

(partial model without unconserved sides chains): PDB file : Tito_1EZW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EZW-query.scw PDB file : Tito_Scwrl_1EZW.pdb: