Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKISFSESQKLFAYFSGLIAALSLFIYYVSAQQSEGALILCITFGVIAAGIWFGPIYALAVTLIVLFVLGTLMMFFQTGQTSLFPAEEGLRMLVVWGIALLLFSFISGRIHDITAELRRSMTRLQSEIKSYVAVDRVTGFDNKQRMKLELSEEIKRAERYGNSFVFLLLHMHYFKEFKSLYGEKETDRLFQYVGQQIRTSVRETDKKFRPSDERIGIVLTHTP-AEHMPAVLTKLKKQLDTYQLE-NGKYVSLTFHVCYLPYRNDIQTADQFLEELENEMMMNEL 4IOB Chain:A ((1-146)) ----------------------------------------------------------------------------------------------------------------------------------------LTSLPNRAFFEGRLSRALRDANEHREQLAVLFIDSDRFKEINDRLGHAAGDTVLVNIAMRIRGQLRESDLVARLGGDEFAVLLAPLASGADALRIADNIIASMQAPIRLSDGSTVSTSLTIGIALYPEHADTPAALLHDADMAMYI---

General information: TITO was launched using: RESULT: Template: 4IOB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 647 -100015 -154.58 -694.55 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -154.58 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.646

(partial model without unconserved sides chains): PDB file : Tito_4IOB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IOB-query.scw PDB file : Tito_Scwrl_4IOB.pdb: