Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIQVEENEHIQTLVYQLNKEGKSICGDSFFMKAD-DKELICAVADGLGSGSLANESSAAIKDLVENYAS------EDVESIIERCNQAMKN-----------KRGATASILKINFEQRQFTYCSVGNVRFILHSPSGESFYPLPISGY--------------------------------LS-------GKPQKYKTHTATY--EKGSKFIIHTDGLNVPDIRSHLK----KGQSVEEISNSLKMYT--TSRKDDLTYILGQLS 4DA1 Chain:A ((133-375)) ---------------------RKENEDRFDFAQLTDEVLYFAVYDGHGGPAAADFCHTHMEKCIMDLLPKEKNLETLLTLAFLEIDKAFSSHARLSADATLLTSGTTATVALLRD-GIELVVASVGDSRAILCRKG-KPMKLTIDHTPERKDEKERIKKCGGFVAWNQPHVNGRLAMTRSIGDLDLKTSGVIAEPETKRIKLHHADDSFLVLTTDGINFMVNSQEICDFVNQCHDPNEAAHAVTEQAIQYGTEDNSTAVVVPF-

General information: TITO was launched using: RESULT: Template: 4DA1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 996 -111930 -112.38 -639.60 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -112.38 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.351

(partial model without unconserved sides chains): PDB file : Tito_4DA1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4DA1-query.scw PDB file : Tito_Scwrl_4DA1.pdb: