Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTNFILNENKQLSLAIEDENIENFYIDGTDLVRKIIRRSGSGVTSRVPVLSTQDLENKNLHELYDESWLRMKNRPNTELTTESINIADLFSGCGGLSLGVWEACRALGINPRFSFACDLNEAALSVYEKNFSPDFSLNESIEKHINGELGAPLTVEEQRIKDKVKKIDFILAGPPCQGHSDLNNHTRRKDPRNALLMRVSRVIELFQPSSVLVENVPGIIHDKSGSFKEFKNHLKTQGYYF-DEIVLNAEKLGVSQARRRYFIFASKTPVSSLN---QINEFYSTNSRPISWAISDLVENVGDDIFN-T--ASEHSLENK----R-RIEYLFE--NNLF---ELPNSERPDCHR----------L-----KPHSYKSVYGRMYWDRPAPTITRGFGSTGQGRFVHSLLKRTITPHEAARIQFFPDFFNFGDLRRRQYQDVIGNAVPSKLSYLLALHQLR 3G7U Chain:A ((2-365)) ----------------------------------------------------------------------------------SLNVIDLFSGVGGLSLGAARA------GFDVKMAVEIDQHAINTHAINFPRSLHVQEDVSLLNAEII--------KGFFKNDMPIDGIIGGPPCQGFS--------DDSRNQLYMHFYRLVSELQPLFFLAENVPGIMQEKYSGIRNKAFNLVSGDYDILDPIKVKASDYGAPTIRTRYFFIGVKKSLKLDISDEVFMPK-MIDPVTVKDALYGLPDIIDANWQSDSESWRTIKKDRKGGFYEKLWGQIPRNVGDTESIAKLKNNIISGCTGTLHSKIVQERYASLSFGETDKISRSTRLDPNGFCPTL---------VRPIHPYHPRVITPREAARLQGFPDWFRFHVTKWHSFRQ-IGNSVSPIVAEYILKGLYN

General information: TITO was launched using: RESULT: Template: 3G7U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1768 -121657 -68.81 -389.93 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -68.81 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.288

(partial model without unconserved sides chains): PDB file : Tito_3G7U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3G7U-query.scw PDB file : Tito_Scwrl_3G7U.pdb: